在显微光谱系统的相关技术当中,成像光谱技术和显微技术是相结合起来的,两者相互综合而发挥出更大的优越性从而组成了显微光谱系统,为了能够保证该系统能够达到预先使用的效果和目的,所以需要对其技术指标有所了解,只有达到以下几个技术指标的产品才能够达到质量要求。
一、光谱范围
显微角分辨光谱公司介绍因为一般来说光谱范围需要能够包括所处理的样品的特性,并且还应该根据分工方式来确定。为了所选用的显微光谱系统可以相关物体的光伏情况,所以有必要对光谱范围做一定的要求,并且还可根据反射光吸收光谱范围以及所激发的荧光光谱范围综合确定工作光谱范围。
二、谱段数
在选取显微光谱系统的谱段数的时候,需要先弄清楚CCD面阵相机的光谱像元数,所以是没有特定标准的。不过对某些品牌的显微光谱系统来说,也可以根据以往的经验来选择合理的范围,比如有些操作者会选择谱段数为2的整数次幂等等。
三、光谱分辨率
显微光谱系统的光谱分辨率是比较重要的一个参数和技术指标,所以应该要对此了解清楚,并且它主要和分工系统的设计有着不可分割的关系,而且如果是色散型的风光系统那么,它还跟两者之间的夹缝宽度有关系。所以要从这些方面出发去进行辨别显微光谱系统的技术情况,其中狭缝的宽窄和光谱分辨率成反比,并且同时还应该要考虑到实际应用的问题。
以上就是想为光谱系统的主要技术指标所包括的几个方面,另外,还有空间分辨率也是其中的参数之一。这主要由显微物镜的数值孔径以及放大倍率和CCD像元大小而定,并且在采用激光扫描共焦荧光显微光谱系统中,这种设备比普通显微系统的空间分辨率提高很多。
Among the related technologies of microspectral system, imaging spectrum technology and microspectral technology are combined. They combine with each other to give full play to their greater advantages and thus constitute a microspectral system. In order to ensure that the system can achieve the effect and purpose of pre-use, it is necessary to understand its technical indicators. Only products that achieve the following technical indicators are needed To meet the quality requirements.
1、 Spectral range
In general, the spectrum range needs to be able to include the characteristics of the processed samples, and should also be determined according to the division of labor. In order to select the microspectral system that can be related to the photovoltaic situation of the object, it is necessary to make certain requirements for the spectral range, and also can determine the working spectral range comprehensively according to the absorption spectral range of reflected light and the excited fluorescence spectral range.
2、 Number of spectral segments
When selecting the number of spectral segments of the microspectral system, we need to know the number of spectral pixels of CCD array camera first, so there is no specific standard. However, for some brands of microspectral systems, we can also choose a reasonable range based on previous experience, for example, some operators will choose the integral power of the number of spectral segments as 2, and so on.
3、 Spectral resolution
The spectral resolution of microspectral system is an important parameter and technical index, so it should be understood clearly, and it mainly has an inseparable relationship with the design of the division system, and if it is a dispersive system, it also has a relationship with the gap width between them. Therefore, it is necessary to identify the microspectral system from these aspects, in which the width of the slit is inversely proportional to the spectral resolution, and at the same time, the practical application should be considered.
The above are the main technical specifications of the spectral system, in addition, the spatial resolution is also one of the parameters. This is mainly determined by the numerical aperture, magnification and CCD pixel size of the microscope objective. In the laser scanning confocal fluorescence microscope system, the spatial resolution of this equipment is much higher than that of the ordinary microscope system.